Microsart® RESEARCH Bacteria
Rapid detection of total bacteria within 2½ h.
User Benefits
- >95 % of all known bacteria detected in one test
- fast: only 2½ h time-to-result
- reliable: highly specific TaqMan® probes
- easy to use
- less pipetting effort
- no sample preparation mandatory
Product Information
Microsart® RESEARCH Bacteria is used for fast and reliable direct detection of bacterial contamination in cell cultures, cell culture supernatants and cell media components in research and development or whenever there is no need for regulation conform testing (i.e. according to EP/USP/JP).
Kit Components and storage
Each kit contains all reagents for 25 or 100 reactions. 4 colorcoded tubes, with master mix, buffer, positive control and negative control, make the handling as simple as possible.
The expiry date and the storage conditions of the unopened package are noted on the package label. The kit components are stored until use at +2°C to +8°C and must be stored after rehydration or opening at < -18°C.
Please note: The master mix, also called RESEARCH Bacteria Mix, should be protected from light all the time.
Test Principle
Microsart® RESEARCH Bacteria utilizes real-time PCR. The detection procedure can be performed within 2½ hours, including less than ½ hour hands-on time. In contrast to the detection by cell cultivation method, samples do not need to contain vital bacteria.
The assay can be performed with any type of real-time PCR cycler able to detect the fluorescence dyes FAM and ROX.
