SuperScript® III Reverse Transcriptase

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. Like SuperScript® II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. SuperScript® III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability. SuperScript® III RT features:

• Thermostability—half-life of 220 min at 50°C
• Yield—reduced RNase H activity for more full-length cDNA
• Specificity—full activity at 50°C for increased specificity with gene-specific primers
• Applications—array labeling, cDNA libraries, RT-PCR, primer extension, 3´ and 5´ RACE

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